Product Name: IL-3B Antibody (biotin)
Species Reactivity: Rat
Tested Applications: ELISA, WB
Applications: ELISA:To detect IL-3-β by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant IL-3-β.Sandwich:To detect Rat IL-3β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-3β (XP-5187) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-3β. Western Blot:To detect IL-3-β by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-3-β is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat IL-3-β (rat IL-3-β).
Host Species: Goat
Purification: Anti-IL-3-β specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 34161-24-5
Product: Eicosapentaenoic acid (ethyl ester)
Buffer:
Concentration:
Storage Conditions: IL-3B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names:
Accession NO.: P97688
Protein Ino: 81870605
Official Symbol: Il3
Geneid: 24495
Background: IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells
PubMed ID:http://aac.asm.org/content/26/4/493.abstract
