Product Name: IL-1B Antibody (biotin)
Species Reactivity: Mouse
Tested Applications: ELISA, WB
Applications: ELISA:To detect mIL-1-β by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-1- β. Sandwich:To detect mIL-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-1β (XP-5179) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-1β.Western Blot:To detect mIL-1- β by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1- β is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1- β (murine Interleukin-1- β).
Host Species: Rabbit
Purification: Anti-mIL-1- β specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 4008-48-4
Product: LOXO-101 (sulfate)
Buffer:
Concentration:
Storage Conditions: IL-1B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: Il-1b, IL-1betaInterleukin-1 betaIL-1 beta
Accession NO.: P10749
Protein Ino: 124304
Official Symbol: Il1b
Geneid: 16176
Background:
PubMed ID:http://aac.asm.org/content/26/2/270.abstract
