Product Name: IgM Antibody [MuHC2]
Species Reactivity: Human
Tested Applications: IF, IHC, WB
Applications: Immunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Human Mu heavy chain was used as the immunogen for this IgM antibody.
Host Species: Mouse
Purification: Protein G
Physical State: Liquid
CAS NO.: 51411-04-2
Product: Pleconaril
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names:
Accession NO.:
Protein Ino:
Official Symbol: IGHM
Geneid: 3507
Background: IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.
PubMed ID:http://aac.asm.org/content/25/5/633.abstract
