Product Name :
Prestained Protein Ladder (3.5 – 245 kDa)
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As indicated on product label or CoA when stored as recommended. Store at 4°C for 3 months. Store at -20°C for 24 months.
Description:
Protein ladder for use with protein gel electrophoresis 3 µL or 5 µL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.Three color protein standard with 13 prestained proteins.Apply more for thicker (>1.5 mm) or larger gel. Enzo’s Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).Our Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.{{2873460-70-7} site|{2873460-70-7} Technical Information|{2873460-70-7} Data Sheet|{2873460-70-7} supplier} The ladder is supplied in gel loading buffer and is ready to use.{{7738-22-9} MedChemExpress|{7738-22-9} Technical Information|{7738-22-9} References|{7738-22-9} manufacturer} Do not heat, dilute or add reducing agent before loading.PMID:30000866 Guide for Molecular Weight Estimation (kDa): Migration patterns of Prestained Protein Ladder in different electrophoresis conditions. Guide for Molecular Weight Estimation (kDa): Migration patterns of Prestained Protein Ladder in different electrophoresis conditions.
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| Formulation Approximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25°C), 2% SDS, 0.2mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).
