anti-histone h3 (trimethyl lys4, serotonyl gln5) rabbit pab

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Anti-Histone H3 (Trimethyl Lys4, Serotonyl Gln5) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K4me3/Q5ser Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K4me3/Q5ser UniProt ID P68431 Immunogen Trimethylated/serotonylated human histone H3 (Lys4/Gln5) peptide MW (kDa) 15 Specificity Anti-Trimethyl/Serotonyl-Histone H3 (Lys4/Gln5) Rabbit pAb detects histone H3 only when it is Trimethylated at Lys4and serotonylated at Gln5. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. Serotonin, also known as 5-hydroxytryptamine (5-HT), is an excitatory neurotransmitter that regulates brain cell activity by activating specific membrane receptors in the brain. The amino group of serotonins can covalently bind to the glutamine residue at the fifth position of histone H3 by TGM2 to form histone 5-hydroxytryptamine (H3Q5ser). H3Q5ser modifies sites adjacent to H3K4me3 form PTM crosstalk. The presence of H3K4me3Q5ser enhanced the binding of transcription factors, such as TFIID to chromatin, thereby activating the expression of downstream genes in developing rodent brains and human neurons. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRL, Mouse kidneyProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: lane 1: :
Anti-Histone H3 (Trimethyl Lys4, Serotonyl Gln5) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K4me3/Q5ser Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K4me3/Q5ser UniProt ID P68431 Immunogen Trimethylated/serotonylated human histone H3 (Lys4/Gln5) peptide MW (kDa) 15 Specificity Anti-Trimethyl/Serotonyl-Histone H3 (Lys4/Gln5) Rabbit pAb detects histone H3 only when it is Trimethylated at Lys4and serotonylated at Gln5. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. Serotonin, also known as 5-hydroxytryptamine (5-HT), is an excitatory neurotransmitter that regulates brain cell activity by activating specific membrane receptors in the brain. The amino group of serotonins can covalently bind to the glutamine residue at the fifth position of histone H3 by TGM2 to form histone 5-hydroxytryptamine (H3Q5ser). H3Q5ser modifies sites adjacent to H3K4me3 form PTM crosstalk. The presence of H3K4me3Q5ser enhanced the binding of transcription factors, such as TFIID to chromatin, thereby activating the expression of downstream genes in developing rodent brains and human neurons. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRL, Mouse kidneyProtein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: lane 1: