anti-histone h2b rabbit pab

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Anti-Histone H2B Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone H2B type 1-C/E/F/G/I, Histone H2B.1A, H2BC4, H2BFL, HIST1H2BC, Histone H2B.a (H2B/a), Histone H2B.g (H2B/g), Histone H2B.h (H2B/h) Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone H2B type 1-C/E/F/G/I, Histone H2B.1A, H2BC4, H2BFL, HIST1H2BC, Histone H2B.a (H2B/a), Histone H2B.g (H2B/g), Histone H2B.h (H2B/h) UniProt ID P62807 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H2B protein MW (kDa) 14 Specificity Anti-Histone H2B Rabbit pAb detects endogenous levels of total histone H2B protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone H2B is primarily acetylated at Lys5, 12, 15, and 20, and phosphorylated at Ser14. Mono-ubiquitination of histone H2B at Lys120 triggers downstream lysine methylation on histone H3 and introduces changes in chromatin structure. Cellular location Nucleus Images WB Lysate: HeLa, COS-7, BRL, N2a, rH2BProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Anti-Histone H2B Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone H2B type 1-C/E/F/G/I, Histone H2B.1A, H2BC4, H2BFL, HIST1H2BC, Histone H2B.a (H2B/a), Histone H2B.g (H2B/g), Histone H2B.h (H2B/h) Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone H2B type 1-C/E/F/G/I, Histone H2B.1A, H2BC4, H2BFL, HIST1H2BC, Histone H2B.a (H2B/a), Histone H2B.g (H2B/g), Histone H2B.h (H2B/h) UniProt ID P62807 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H2B protein MW (kDa) 14 Specificity Anti-Histone H2B Rabbit pAb detects endogenous levels of total histone H2B protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone H2B is primarily acetylated at Lys5, 12, 15, and 20, and phosphorylated at Ser14. Mono-ubiquitination of histone H2B at Lys120 triggers downstream lysine methylation on histone H3 and introduces changes in chromatin structure. Cellular location Nucleus Images WB Lysate: HeLa, COS-7, BRL, N2a, rH2BProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with