anti-hip1 mouse mab

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Recombinant Anti-HIP1 Mouse mAb Clone Number: JMMR-2033 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: Huntingtin-interacting protein 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Huntingtin-interacting protein 1 UniProt ID O00291 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HIP1 plays a role in clathrin-mediated endocytosis and trafficking, which is involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. It regulates presynaptic nerve terminal activity. It can enhance androgen receptor (AR)-mediated transcription and may act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. It can bind 3-phosphoinositides (via ENTH domain). It is required for differentiation, proliferation, and/or survival of somatic and germline progenitors. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: A549, 2: HCT 116, 3: Neuro-2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 116 kDa Observed band size: 116 kDa ICC/IF Cell line: Neuro-2a Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-HIP1 Mouse mAb Clone Number: JMMR-2033 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: Huntingtin-interacting protein 1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Huntingtin-interacting protein 1 UniProt ID O00291 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HIP1 plays a role in clathrin-mediated endocytosis and trafficking, which is involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. It regulates presynaptic nerve terminal activity. It can enhance androgen receptor (AR)-mediated transcription and may act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. It can bind 3-phosphoinositides (via ENTH domain). It is required for differentiation, proliferation, and/or survival of somatic and germline progenitors. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: A549, 2: HCT 116, 3: Neuro-2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 116 kDa Observed band size: 116 kDa ICC/IF Cell line: Neuro-2a Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with