anti-hec1 mouse mab

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Recombinant Anti-HEC1 Mouse mAb Clone Number: JMMR-2717 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: HsHec1, NDC80, HEC, KNTC2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HsHec1, NDC80, HEC, KNTC2 UniProt ID O14777 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HEC1 is one of the proteins of outer kinetochore which has microtubule-binding domains. It forms a heterotetramer with proteins NUF2, SPC25, and SPC24. HEC1 acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. It Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. Cellular location Nucleus, Kinetochore Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: HEK-293TProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 74 kDaObserved band size: 74 kDa :
Recombinant Anti-HEC1 Mouse mAb Clone Number: JMMR-2717 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: HsHec1, NDC80, HEC, KNTC2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HsHec1, NDC80, HEC, KNTC2 UniProt ID O14777 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HEC1 is one of the proteins of outer kinetochore which has microtubule-binding domains. It forms a heterotetramer with proteins NUF2, SPC25, and SPC24. HEC1 acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. It Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. Cellular location Nucleus, Kinetochore Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: HEK-293TProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 74 kDaObserved band size: 74 kDa