anti-glut3 + glut14 rabbit mab

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Recombinant Rabbit Monoclonal Anti-GLUT3 + GLUT14 Rabbit mAb Clone Number: JMMR-3181 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Rat Synonyms: SLC2A3, GTR3, SLC2A14, GTR14 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms SLC2A3, GTR3, SLC2A14, GTR14 UniProt ID P11169 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Glucose transporter 3 (Glut3), also known as SLC2A3, has a higher affinity for glucose than the ubiquitous glucose transporter Glut1. In the normal brain, Glut3 expression is limited to neurons, whereas Glut1 is expressed in both neurons and glia. Glioblastoma-derived brain tumor initiating cells (BTICs) express increased levels of Glut3, enabling their survival in the microenvironment deficient in glucose. In addition, Glut3 expression levels correlate with poor survival in brain tumors. Glucose transporter 4 (Glut4) is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation. Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking.The antibody can detect both GLUT3 and GLUT4. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: U-87 MG, 2: HepG2, 3: C6 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 54 kDa Observed band size: 54 kDa :
Recombinant Rabbit Monoclonal Anti-GLUT3 + GLUT14 Rabbit mAb Clone Number: JMMR-3181 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Rat Synonyms: SLC2A3, GTR3, SLC2A14, GTR14 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms SLC2A3, GTR3, SLC2A14, GTR14 UniProt ID P11169 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Glucose transporter 3 (Glut3), also known as SLC2A3, has a higher affinity for glucose than the ubiquitous glucose transporter Glut1. In the normal brain, Glut3 expression is limited to neurons, whereas Glut1 is expressed in both neurons and glia. Glioblastoma-derived brain tumor initiating cells (BTICs) express increased levels of Glut3, enabling their survival in the microenvironment deficient in glucose. In addition, Glut3 expression levels correlate with poor survival in brain tumors. Glucose transporter 4 (Glut4) is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation. Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking.The antibody can detect both GLUT3 and GLUT4. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: U-87 MG, 2: HepG2, 3: C6 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 54 kDa Observed band size: 54 kDa