anti-gelsolin rabbit mab

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Recombinant Rabbit Monoclonal Anti-Gelsolin Rabbit mAb Clone Number: JRMR-11059-66B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: AGEL, Actin-depolymerizing factor (ADF), Brevin Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms AGEL, Actin-depolymerizing factor (ADF), Brevin UniProt ID P06396 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:100 – 1:1000 Human, Rat, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Gelsolin also named as actin-depolymerizing factor, ADF, AGEL or Brevin, is an 83 kDa protein that shares structural and functional homology to villin and adseverin/scinderin. Gelsolin plays an important role in actin filament assembly by capping and severing actin proteins in a Ca2+-dependent manner. Gelsolin is important for cellular events (e.g., membrane ruffling, chemotaxis, ciliogenesis) that require cytoskeletal remodeling. Accordingly, cells from gelsolin knockout mice exhibit motility defects, including a failure to ruffle in response to growth factor stimulation. In humans, defects in gelsolin have been linked to amyloidosis type 5 (AMYL5), a hereditary disease characterized by cranial neuropathy, which appears to result from gelsolin amyloid deposition. Cellular location Cytoplasm, cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa; 2: C2C12; 3: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 85 kDaObserved band size: 82 kDa :
Recombinant Rabbit Monoclonal Anti-Gelsolin Rabbit mAb Clone Number: JRMR-11059-66B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: AGEL, Actin-depolymerizing factor (ADF), Brevin Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms AGEL, Actin-depolymerizing factor (ADF), Brevin UniProt ID P06396 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:100 – 1:1000 Human, Rat, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Gelsolin also named as actin-depolymerizing factor, ADF, AGEL or Brevin, is an 83 kDa protein that shares structural and functional homology to villin and adseverin/scinderin. Gelsolin plays an important role in actin filament assembly by capping and severing actin proteins in a Ca2+-dependent manner. Gelsolin is important for cellular events (e.g., membrane ruffling, chemotaxis, ciliogenesis) that require cytoskeletal remodeling. Accordingly, cells from gelsolin knockout mice exhibit motility defects, including a failure to ruffle in response to growth factor stimulation. In humans, defects in gelsolin have been linked to amyloidosis type 5 (AMYL5), a hereditary disease characterized by cranial neuropathy, which appears to result from gelsolin amyloid deposition. Cellular location Cytoplasm, cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa; 2: C2C12; 3: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 85 kDaObserved band size: 82 kDa