anti-gclc rabbit mab

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Recombinant Rabbit Monoclonal Anti-GCLC Rabbit mAb Clone Number: JMMR-3671 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Rat Synonyms: GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC UniProt ID P48506 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Rat Properties Purity Protein A purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Glutamate-cysteine ligase catalytic subunit (GCLC) catalyzes the ligation of glutamate and cysteine, the first and rate-limiting step in glutathione biosynthesis. Studies show that deubiquitinases protect cancer cells when glutathione synthesis is suppressed. Upon inhibition of both GCLC and deubiquitinases, cancer cells undergo proteotoxic stress, leading to cell death. In addition, deletion of Gclc enhances reactive oxygen species in mouse regulatory T cells (Tregs). Glutathione synthesized in part by Gclc is required for the suppressive function of mouse Tregs. Furthermore, in non-small cell lung cancer (NSCLC) cell lines, the transcription factor NRF2 stimulates GCLC expression. When NSCLC cells are starved for cysteine, GCLC, through its non-canonical activity, catalyzes the synthesis of γ-glutamyl-peptide, therefore reducing glutamate levels to protect cells against ferroptosis. Cellular location Cytoplasm, Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: A549, 2: HepG2, 3: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 73 kDa Observed band size: 73 kDa :
Recombinant Rabbit Monoclonal Anti-GCLC Rabbit mAb Clone Number: JMMR-3671 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Rat Synonyms: GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC UniProt ID P48506 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human, Rat Properties Purity Protein A purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Glutamate-cysteine ligase catalytic subunit (GCLC) catalyzes the ligation of glutamate and cysteine, the first and rate-limiting step in glutathione biosynthesis. Studies show that deubiquitinases protect cancer cells when glutathione synthesis is suppressed. Upon inhibition of both GCLC and deubiquitinases, cancer cells undergo proteotoxic stress, leading to cell death. In addition, deletion of Gclc enhances reactive oxygen species in mouse regulatory T cells (Tregs). Glutathione synthesized in part by Gclc is required for the suppressive function of mouse Tregs. Furthermore, in non-small cell lung cancer (NSCLC) cell lines, the transcription factor NRF2 stimulates GCLC expression. When NSCLC cells are starved for cysteine, GCLC, through its non-canonical activity, catalyzes the synthesis of γ-glutamyl-peptide, therefore reducing glutamate levels to protect cells against ferroptosis. Cellular location Cytoplasm, Mitochondrion Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: A549, 2: HepG2, 3: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 73 kDa Observed band size: 73 kDa