anti-gapdh mouse mab

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Recombinant Anti-GAPDH Mouse mAb Clone Number: JMMR-2818 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat, A.thaliana Synonyms: GAPD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms GAPD UniProt ID P04406 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:10000 Human, Mouse, Rat, Arabidopsis thaliana IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background GAPDH is a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). GAPDH is usually used as a loading control for Western blot. Cellular location Cytoplasm, Cytoskeleton, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa 2: NIH/3T3, 3: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 36 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Arabidopsis Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 36 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-GAPDH Mouse mAb Clone Number: JMMR-2818 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat, A.thaliana Synonyms: GAPD Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms GAPD UniProt ID P04406 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:10000 Human, Mouse, Rat, Arabidopsis thaliana IHC-P 1:50 – 1:100 Human, Mouse, Rat ICC/IF 1:50 Human FC 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background GAPDH is a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). GAPDH is usually used as a loading control for Western blot. Cellular location Cytoplasm, Cytoskeleton, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa 2: NIH/3T3, 3: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 36 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Arabidopsis Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 36 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with