anti-acetyl-histone h3 (lys9) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb Clone Number: PA-169-56 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ChIP CUT&Tag Reactivity: Human, Mouse, Rat Synonyms: H3K9ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K9ac UniProt ID P68431 Immunogen Acetylated human histone H3 (Lys9) peptide MW (kDa) 15 Specificity Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb detects histone H3 only when it is acetylated at Lys9. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys9, but not the structurally similar butyrylated, β-hydroxybutyrylated, or 2-hydroxyisobutyrylated peptide at Lys9 or Lys27. The antibody does not cross-react with other acetylated H3 peptides at Lys4, Lys14, or Lys27. Product Usage Information Applications Dilution Recommended Species WB 1:5000 – 1:10000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human CUT&Tag 1:20 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 3 seconds Predicted band size: 15 kDa Observed band size: 15 kDa Lysate: (-): NIH/3T3; (+): NIH/3T3 + sodium butyrate (500 ng/ml, 4 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 3 seconds Predicted band size: 15 kDa Observed band size: 15 kDa Lysate: (-): C6; (+): C6 + sodium butyrate (500 ng/ml, 5 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human kidney Section type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb Clone Number: PA-169-56 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ChIP CUT&Tag Reactivity: Human, Mouse, Rat Synonyms: H3K9ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K9ac UniProt ID P68431 Immunogen Acetylated human histone H3 (Lys9) peptide MW (kDa) 15 Specificity Anti-Acetyl-Histone H3 (Lys9) Rabbit mAb detects histone H3 only when it is acetylated at Lys9. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys9, but not the structurally similar butyrylated, β-hydroxybutyrylated, or 2-hydroxyisobutyrylated peptide at Lys9 or Lys27. The antibody does not cross-react with other acetylated H3 peptides at Lys4, Lys14, or Lys27. Product Usage Information Applications Dilution Recommended Species WB 1:5000 – 1:10000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human CUT&Tag 1:20 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 3 seconds Predicted band size: 15 kDa Observed band size: 15 kDa Lysate: (-): NIH/3T3; (+): NIH/3T3 + sodium butyrate (500 ng/ml, 4 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 3 seconds Predicted band size: 15 kDa Observed band size: 15 kDa Lysate: (-): C6; (+): C6 + sodium butyrate (500 ng/ml, 5 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds Predicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human kidney Section type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with