anti-gaba b receptor 1 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-GABA B Receptor 1 Rabbit mAb Clone Number: JRMR-10463-1004 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Mouse, Rat Synonyms: GABA-B-R1, GABA-BR1, GABABR1, Gb1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms GABA-B-R1, GABA-BR1, GABABR1, Gb1 UniProt ID Q9UBS5 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Rat, Mouse IHC-P 1:200 – 1000 Rat, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site. GABA(B)R1 has two isoforms: GABA(B)R1a and GABA(B)R1b. GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein. G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Mouse skeletal muscle(-), 2: Mousecerebellum, 3: Rat skeletal muscle(-), 4: Rat cerebellumProtein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 108 kDa Observed band size: 108 kDa IHC-P Tissue: Mouse cerebellumSection type: Formalin-fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-GABA B Receptor 1 Rabbit mAb Clone Number: JRMR-10463-1004 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Mouse, Rat Synonyms: GABA-B-R1, GABA-BR1, GABABR1, Gb1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms GABA-B-R1, GABA-BR1, GABABR1, Gb1 UniProt ID Q9UBS5 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Rat, Mouse IHC-P 1:200 – 1000 Rat, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site. GABA(B)R1 has two isoforms: GABA(B)R1a and GABA(B)R1b. GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein. G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Mouse skeletal muscle(-), 2: Mousecerebellum, 3: Rat skeletal muscle(-), 4: Rat cerebellumProtein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 108 kDa Observed band size: 108 kDa IHC-P Tissue: Mouse cerebellumSection type: Formalin-fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with