anti-fatty acid synthase rabbit mab

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Recombinant Rabbit Monoclonal Anti-Fatty Acid Synthase Rabbit mAb Clone Number: JRMR-10144-3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC IP Reactivity: Human, Mouse, Rat Synonyms: Type I fatty acid synthase, FASN Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Type I fatty acid synthase, FASN UniProt ID P49327 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human, Mouse, Rat FC 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4′-phosphopantetheine of the acyl carrier protein ([ACP]) domain. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: HeLa, 293T, A549, HepG2, MOLT-4, PC-3 Protein loading quantity: 20 μgExposure time: 60 secondsPredicted band size: 273 kDa Observed band size: 260 kDa IHC-P Tissue: Human liverSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Fatty Acid Synthase Rabbit mAb Clone Number: JRMR-10144-3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC IP Reactivity: Human, Mouse, Rat Synonyms: Type I fatty acid synthase, FASN Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Type I fatty acid synthase, FASN UniProt ID P49327 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human, Mouse, Rat FC 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4′-phosphopantetheine of the acyl carrier protein ([ACP]) domain. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: HeLa, 293T, A549, HepG2, MOLT-4, PC-3 Protein loading quantity: 20 μgExposure time: 60 secondsPredicted band size: 273 kDa Observed band size: 260 kDa IHC-P Tissue: Human liverSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with