anti-eif3l mouse mab

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Recombinant Anti-eIF3L Mouse mAb Clone Number: 11963 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: EIF3EIP, EIF3S6IP Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms EIF3EIP, EIF3S6IP UniProt ID Q9Y262 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse ICC/IF 1:50 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate:1: 293, 2: Raw264.7, 3: 3T3, 4: HepG2Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 67 kDaObserved band size: 55-70 kDa ICC/IF Cell line: HEK-293Fixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Anti-eIF3L Mouse mAb Clone Number: 11963 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: EIF3EIP, EIF3S6IP Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms EIF3EIP, EIF3S6IP UniProt ID Q9Y262 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse ICC/IF 1:50 – 1:200 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate:1: 293, 2: Raw264.7, 3: 3T3, 4: HepG2Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 67 kDaObserved band size: 55-70 kDa ICC/IF Cell line: HEK-293Fixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with