anti-eif2s1 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-EIF2S1 Rabbit mAb Clone Number: JRMR-10438-34 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: EIF2S1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms EIF2S1 UniProt ID P05198 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The eukaryotic translation initiation factor 2-α subunit (EIF2S1) is the first regulatory step that catalyzes the initiation of protein synthesis and promotes the binding of the initial tRNA to the 40S ribosomal subunit. As an important translation initiation factor, the eukaryotic initiation factor 2-α subunit is regulated by a variety of signal molecules, that is, it functions by regulating the phosphorylation and acetylation status of EIF2S1. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate EIF2S1 to regulate the transcription and translation of cell-related genes and enhance Cell protection mechanism. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: HepG2, 3: MCF-7, 4: Raw264.7, 5: C6Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 36 kDaObserved band size: 36 kDa IHC-P Tissue: Human breastSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-EIF2S1 Rabbit mAb Clone Number: JRMR-10438-34 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: EIF2S1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms EIF2S1 UniProt ID P05198 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The eukaryotic translation initiation factor 2-α subunit (EIF2S1) is the first regulatory step that catalyzes the initiation of protein synthesis and promotes the binding of the initial tRNA to the 40S ribosomal subunit. As an important translation initiation factor, the eukaryotic initiation factor 2-α subunit is regulated by a variety of signal molecules, that is, it functions by regulating the phosphorylation and acetylation status of EIF2S1. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate EIF2S1 to regulate the transcription and translation of cell-related genes and enhance Cell protection mechanism. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: HepG2, 3: MCF-7, 4: Raw264.7, 5: C6Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 36 kDaObserved band size: 36 kDa IHC-P Tissue: Human breastSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with