anti-eef1a1 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-eEF1A1 Rabbit mAb Clone Number: JMMR-1828B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: EF-Tu, EF-1-alpha-1, EEF1A, EF1A, LENG7 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms EF-Tu, EF-1-alpha-1, EEF1A, EF1A, LENG7 UniProt ID P68104 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background eEF1A, also named elongation factor Tu (EF-Tu), is one of the eukaryotic elongation factors (eEFs), that function in translation. eEF1A binds GTP and interacts with amino acyl-tRNAs to promote the recruitment of amino acyl-tRNAs to the A-site of the ribosome. After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B. eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level. Cellular location Cytosol, Nucleus, Plasma membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: MCF7, 2: PANC-1, 3: Neuro-2a, 4: Rat kidneyProtein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 50 kDa Observed band size: 55 kDa ICC/IF Cell line: MCF7 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°Covernight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-eEF1A1 Rabbit mAb Clone Number: JMMR-1828B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: EF-Tu, EF-1-alpha-1, EEF1A, EF1A, LENG7 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms EF-Tu, EF-1-alpha-1, EEF1A, EF1A, LENG7 UniProt ID P68104 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background eEF1A, also named elongation factor Tu (EF-Tu), is one of the eukaryotic elongation factors (eEFs), that function in translation. eEF1A binds GTP and interacts with amino acyl-tRNAs to promote the recruitment of amino acyl-tRNAs to the A-site of the ribosome. After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B. eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level. Cellular location Cytosol, Nucleus, Plasma membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: MCF7, 2: PANC-1, 3: Neuro-2a, 4: Rat kidneyProtein loading quantity: 20 μg Exposure time: 10 secondsPredicted band size: 50 kDa Observed band size: 55 kDa ICC/IF Cell line: MCF7 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°Covernight Secondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with