Product Name :
anti-e-cadherin rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
CAM 120/8, E-cadherin, CD324
UniProt ID :
P12830
Immunogen:
Recombinant human E-Cadherin fragment
MW (kDa) :
90-120
Specificity:
Anti-E-Cadherin Rabbit mAb recognizes endogeneous level of E-Cadherin protein.
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Cellular location Membrane
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: HeLa (Negative control), 2: T-47D,3: MCF-7, 4: HT-29Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 97 kDaObserved MW: 90-120 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: Mouse stomach, 2: RatstomachProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 97 kDaObserved MW: 90-120 kDa IP IP of MCF-7 cells extractsIP Ab incubation condition:
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-E-Cadherin Rabbit mAb Clone Number: JRMR-10614-48 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IP Reactivity: Human, Mouse, Rat Synonyms: CAM 120/8, E-cadherin, CD324 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CAM 120/8, E-cadherin, CD324 UniProt ID P12830 Immunogen Recombinant human E-Cadherin fragment MW (kDa) 90-120 Specificity Anti-E-Cadherin Rabbit mAb recognizes endogeneous level of E-Cadherin protein. Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:5000 Human, Mouse, Rat IP 1:50-1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: HeLa (Negative control), 2: T-47D,3: MCF-7, 4: HT-29Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 97 kDaObserved MW: 90-120 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: Mouse stomach, 2: RatstomachProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 97 kDaObserved MW: 90-120 kDa IP IP of MCF-7 cells extractsIP Ab incubation condition:
Recombinant Rabbit Monoclonal Anti-E-Cadherin Rabbit mAb Clone Number: JRMR-10614-48 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IP Reactivity: Human, Mouse, Rat Synonyms: CAM 120/8, E-cadherin, CD324 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CAM 120/8, E-cadherin, CD324 UniProt ID P12830 Immunogen Recombinant human E-Cadherin fragment MW (kDa) 90-120 Specificity Anti-E-Cadherin Rabbit mAb recognizes endogeneous level of E-Cadherin protein. Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:5000 Human, Mouse, Rat IP 1:50-1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: HeLa (Negative control), 2: T-47D,3: MCF-7, 4: HT-29Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 97 kDaObserved MW: 90-120 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Lysate: 1: Mouse stomach, 2: RatstomachProtein loading quantity: 20 μgExposure time: 60 sPredicted MW: 97 kDaObserved MW: 90-120 kDa IP IP of MCF-7 cells extractsIP Ab incubation condition:
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