Product Name :
anti-acetyl-histone h3 (lys27) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K27ac
UniProt ID :
P68431
Immunogen:
Acetylated human histone H3 (Lys27) peptide
MW (kDa) :
15
Specificity:
Anti-Acetyl-Histone H3 (Lys27) Rabbit pAb detects histone H3 only when it is acetylated at Lys27. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys27, but not the crotonylated H3 peptide at Lys27 or unmodified peptide.
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus
Images :
Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 17 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:400Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: Pharbitis, N2a, Mouse kidneyProtein loading quantity: 20 μgExposure time: 30 sPredicted MW: 15 kDaObserved MW: 17 kDa IHC-P Tissue: Human breast cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200Primary ab incubation condition: 1 hour at room temperature Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Description: Color brown is the positive signal for
Vapor Pressure :
Anti-Acetyl-Histone H3 (Lys27) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat, Pharbitis Synonyms: H3K27ac Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（7） $ 320 Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb Catalog #
Anti-Acetyl-Histone H3 (Lys27) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat, Pharbitis Synonyms: H3K27ac Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（7） $ 320 Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb Catalog #
Related websites: https://www.medchemexpress.com/antibodies.html
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