anti-dykddddk tag rabbit mab (binds to flag® tag sequence)

Product Name :
anti-dykddddk tag rabbit mab (binds to flag® tag sequence)

Isotype :
IgG

Conjugate :
Unconjugated

Synonyms:
/

UniProt ID :
/

Immunogen:

MW (kDa) :

Specificity:

Purity :
Protein A purified

Purity :
PBS, Glycerol, BSA

Storage :
Store at -20°C. Avoid freeze/thaw cycles.

Stability:
Stable for 12 months from date of receipt/reconstitution.

Background :
The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein’s function. Cellular location /

Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), 2: HEK-293F transfected with PFKFB3 (WT) (58 kDa) expression vector containing a Flag-tag, whole cell lysateProtein loading quantity: 20 μgExposure time: 30 sObserved band size: 58 kDa

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-DYKDDDDK Tag Rabbit mAb (Binds to FLAG® Tag Sequence) Clone Number: JRMR-10034-3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: / Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 / Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein’s function. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), 2: HEK-293F transfected with PFKFB3 (WT) (58 kDa) expression vector containing a Flag-tag, whole cell lysateProtein loading quantity: 20 μgExposure time: 30 sObserved band size: 58 kDa :
Recombinant Rabbit Monoclonal Anti-DYKDDDDK Tag Rabbit mAb (Binds to FLAG® Tag Sequence) Clone Number: JRMR-10034-3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ChIP Reactivity: / Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 / Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein’s function. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), 2: HEK-293F transfected with PFKFB3 (WT) (58 kDa) expression vector containing a Flag-tag, whole cell lysateProtein loading quantity: 20 μgExposure time: 30 sObserved band size: 58 kDa

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Betaine hydrochloride

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