anti-dimethyl-histone h3 (lys9) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Dimethyl-Histone H3 (Lys9) Rabbit mAb Clone Number: PA-173-35 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K9me2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K9me2 UniProt ID P68431 Immunogen Dimethylated human histone H3 (Lys9) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is Dimethylated at Lys9, but not the Monomethylated, Trimethylated, acetylated and the unmodified H3 peptide at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat ICC/IF 1:100 – 1:200 Human IP 1:25 – 1:100 Human ChIP 6μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation,phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12,16 and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images ELISA Primary Ab dilution: 1:10,000Anti-Dimethyl-Histone H3 (Lys9) Rabbit mAb ( :
Recombinant Rabbit Monoclonal Anti-Dimethyl-Histone H3 (Lys9) Rabbit mAb Clone Number: PA-173-35 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K9me2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K9me2 UniProt ID P68431 Immunogen Dimethylated human histone H3 (Lys9) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is Dimethylated at Lys9, but not the Monomethylated, Trimethylated, acetylated and the unmodified H3 peptide at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat ICC/IF 1:100 – 1:200 Human IP 1:25 – 1:100 Human ChIP 6μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation,phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12,16 and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images ELISA Primary Ab dilution: 1:10,000Anti-Dimethyl-Histone H3 (Lys9) Rabbit mAb (