Product Name :
anti-acetyl-histone h3 (lys18) rabbit pab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K18ac
UniProt ID :
P68431
Immunogen:
Acetylated human histone H3 (Lys18) peptide
MW (kDa) :
15
Specificity:
Anti-Acetyl-Histone H3 (Lys18) Rabbit pAb detects histone H3 only when it is acetylated at Lys18. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys18, but not the structurally similar propionylated, crotonylated, or butyrylated H3 peptides at Lys18.
Purity :
Protein A and immunogen affinity purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus
Images :
Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 17 kDa IP IP of HeLa + sodium butyrate (30 mM, 4 hours) cells extractsLane 1: 5% Input Lane 2: IP with
Vapor Pressure :
Anti-Acetyl-Histone H3 (Lys18) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18ac Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（7） $ 320 Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys18) Rabbit mAb Catalog #
Anti-Acetyl-Histone H3 (Lys18) Rabbit pAb This item is discontinued. Please see alternative products below. Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18ac Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols Alternative Products View More Images（7） $ 320 Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys18) Rabbit mAb Catalog #
Related websites: https://www.medchemexpress.com/antibodies.html
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