anti-dimethyl-histone h3 (lys122) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Dimethyl-Histone H3 (Lys122) Rabbit mAb Clone Number: 76-H1L3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K122me2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K122me2 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Post translational modifications on histones include acetylation, methylation, phosphorylation and some new acylation modifications found in recent years. These histone modifications directly affect the binding of chromatin to transcription factors or other epigenetic regulators, and change genome stability and gene transcription. Histone methylation usually occurs in lysine and arginine residues of core histones. Histone methylation can promote or inhibit gene transcription, depending on whether histone methylation occurs on lysine or arginine and the number of methylation groups (lysine can be monomethyl, dimethyl and trimethylated, arginine can be monomethyl, symmetric and asymmetric dimethyl). Histone lysine methylation usually occurs on lysine 4, 9, 27, 36, 79 of histone H3 and lysine 20 of histone H4; Arginine methylation usually occurs on arginine 2, 8, 17, 26 of histone H3 and arginine 3 of histone H4. Protein methylase (HMT) and histone demethylase (HDM) are the main regulators of methylation modification. H3K122me2 is a newly discovered modification site. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2-4: 64 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: HeLa cytosol, 2: HeLa nuclear Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 15 KDa Observed band size: 15 KDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: Mouse brain, 2: Rat brain, 3: Recombinant histone H3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 15 KDa Observed band size: 15 KD :
Recombinant Rabbit Monoclonal Anti-Dimethyl-Histone H3 (Lys122) Rabbit mAb Clone Number: 76-H1L3 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K122me2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K122me2 UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Post translational modifications on histones include acetylation, methylation, phosphorylation and some new acylation modifications found in recent years. These histone modifications directly affect the binding of chromatin to transcription factors or other epigenetic regulators, and change genome stability and gene transcription. Histone methylation usually occurs in lysine and arginine residues of core histones. Histone methylation can promote or inhibit gene transcription, depending on whether histone methylation occurs on lysine or arginine and the number of methylation groups (lysine can be monomethyl, dimethyl and trimethylated, arginine can be monomethyl, symmetric and asymmetric dimethyl). Histone lysine methylation usually occurs on lysine 4, 9, 27, 36, 79 of histone H3 and lysine 20 of histone H4; Arginine methylation usually occurs on arginine 2, 8, 17, 26 of histone H3 and arginine 3 of histone H4. Protein methylase (HMT) and histone demethylase (HDM) are the main regulators of methylation modification. H3K122me2 is a newly discovered modification site. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2-4: 64 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: HeLa cytosol, 2: HeLa nuclear Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 15 KDa Observed band size: 15 KDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab:1: Mouse brain, 2: Rat brain, 3: Recombinant histone H3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 15 KDa Observed band size: 15 KD