anti-diglycyllysine rabbit pab

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Anti-Diglycyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： IAP Reactivity: All Synonyms: K-ε-GG Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms K-ε-GG UniProt ID / Immunogen Diglycylated lysine peptides MW (kDa) Multiple Specificity Anti-Diglycyl-Lysine Rabbit pAb selectively captures peptides/proteins bearing K-ɛ-G-G residues, which are remnants of ubiquitin left in protein substrates after trypsin digestion, or SUMO remnants generated by α-lytic protease WaLP digestion. This pan antibody recognizes diglycyl-lysine independent of its surrounding sequences and has been well utilized to affinity purify peptides for global proteomic screening of ubiquitination and SUMOylation. Product Usage Information Applications Dilution Recommended Species IAP / All Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation, modulates membrane protein trafficking, alters protein-protein interactions, and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminal Gly76 and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule, resulting in polyubiquitin chains with different functions.Members of the ubiquitin-like protein family, such as small ubiquitin-related modifier 1, 2 and 3 (SUMO1, 2 and 3), have functions similar to ubiquitin, whereby they bind to target proteins as part of a post-translational modification system. SUMO can be covalently attached to proteins as a monomer or a lysine-linked polymer via an isopeptide bond. SUMO-1 regulates nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability by being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. SUMO-2/-3 forms poly-(SUMO) chains and is conjugated to topoisomerase II and APP, regulating chromosomal segregation and cellular responses to environmental stress. Cellular location / Images Dot Blot Peptide/Protein amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides and proteins used in the experiment is provided in the table below. Mass Spectrometry Analysis Representative annotated MS/MS spectra of a ubiquitin remnant-containing peptide. Crude proteins from HeLa cell lysate were digested by trypsin. The ubiquitin remnant-containing peptides were affinity enriched by the Anti-Diglycyl- Lysine Rabbit pAb followed by LC-ESI-MS/MS in a Q-Exactive mass spectrometry. The sequence of the representative ubiquitinated peptide is indicated and the fragment ions are labeled in the MS/MS spectra. The diGly-modified lysine and its position in the peptide sequence are indicated with “gl” above “K”. :
Anti-Diglycyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： IAP Reactivity: All Synonyms: K-ε-GG Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms K-ε-GG UniProt ID / Immunogen Diglycylated lysine peptides MW (kDa) Multiple Specificity Anti-Diglycyl-Lysine Rabbit pAb selectively captures peptides/proteins bearing K-ɛ-G-G residues, which are remnants of ubiquitin left in protein substrates after trypsin digestion, or SUMO remnants generated by α-lytic protease WaLP digestion. This pan antibody recognizes diglycyl-lysine independent of its surrounding sequences and has been well utilized to affinity purify peptides for global proteomic screening of ubiquitination and SUMOylation. Product Usage Information Applications Dilution Recommended Species IAP / All Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation, modulates membrane protein trafficking, alters protein-protein interactions, and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminal Gly76 and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule, resulting in polyubiquitin chains with different functions.Members of the ubiquitin-like protein family, such as small ubiquitin-related modifier 1, 2 and 3 (SUMO1, 2 and 3), have functions similar to ubiquitin, whereby they bind to target proteins as part of a post-translational modification system. SUMO can be covalently attached to proteins as a monomer or a lysine-linked polymer via an isopeptide bond. SUMO-1 regulates nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability by being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. SUMO-2/-3 forms poly-(SUMO) chains and is conjugated to topoisomerase II and APP, regulating chromosomal segregation and cellular responses to environmental stress. Cellular location / Images Dot Blot Peptide/Protein amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides and proteins used in the experiment is provided in the table below. Mass Spectrometry Analysis Representative annotated MS/MS spectra of a ubiquitin remnant-containing peptide. Crude proteins from HeLa cell lysate were digested by trypsin. The ubiquitin remnant-containing peptides were affinity enriched by the Anti-Diglycyl- Lysine Rabbit pAb followed by LC-ESI-MS/MS in a Q-Exactive mass spectrometry. The sequence of the representative ubiquitinated peptide is indicated and the fragment ions are labeled in the MS/MS spectra. The diGly-modified lysine and its position in the peptide sequence are indicated with “gl” above “K”.