anti-diglycyl-lysine antibody conjugated agarose beads

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Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads Clone Number: / Host: / Clonality: / Applications： IAP Reactivity: All Synonyms: K-ε-GG Product Size 500 μl ADD TO CART BUY NOW Quantity Shipping: Cold packs / dry ice Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Agarose Synonyms K-ε-GG UniProt ID / Immunogen Diglycylated lysine peptides MW (kDa) Multiple Specificity With the immobilization of highly specific Anti-Diglycyl-Lysine Antibody, the Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing K-ɛ-G-G residues, which are remnants of ubiquitin left in protein substrates after trypsin digestion or remnants of SUMO after α-lytic protease WaLP digestion. This product has been well utilized to affinity purify peptides for global proteomic screening of ubiquitination and SUMOylation. Product Usage Information Applications Dilution Recommended Species IAP 40 μl beads per 2 mg peptides All Properties Purity / Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody. Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt. Target Information Background Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminus and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule.Members of the ubiquitin-like protein family, such as small ubiquitin-related modifier 1, 2 and 3 (SUMO1, 2 and 3), have functions similar to ubiquitin, whereby they bind to target proteins as part of a post-translational modification system. SUMO can be covalently attached to proteins as a monomer or a lysine-linked polymer via an isopeptide bond. SUMO-1 regulates nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability by being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. SUMO-2/-3 forms poly-(SUMO) chains and is conjugated to topoisomerase II and APP, regulating chromosomal segregation and cellular responses to environmental stress. Cellular location / Images Dot Blot Dot blot analysis of Anti-Diglycyl-Lysine Rabbit pAb ( :
Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads Clone Number: / Host: / Clonality: / Applications： IAP Reactivity: All Synonyms: K-ε-GG Product Size 500 μl ADD TO CART BUY NOW Quantity Shipping: Cold packs / dry ice Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Agarose Synonyms K-ε-GG UniProt ID / Immunogen Diglycylated lysine peptides MW (kDa) Multiple Specificity With the immobilization of highly specific Anti-Diglycyl-Lysine Antibody, the Anti-Diglycyl-Lysine Antibody Conjugated Agarose Beads selectively capture peptides/proteins bearing K-ɛ-G-G residues, which are remnants of ubiquitin left in protein substrates after trypsin digestion or remnants of SUMO after α-lytic protease WaLP digestion. This product has been well utilized to affinity purify peptides for global proteomic screening of ubiquitination and SUMOylation. Product Usage Information Applications Dilution Recommended Species IAP 40 μl beads per 2 mg peptides All Properties Purity / Constituents Antibody conjugated agarose beads are supplied as a 50% slurry containing 66% glycerol and 4 mg/ml immobilized antibody. Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt. Target Information Background Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating cellular processes. By covalently attaching to target proteins through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitination marks the target proteins for proteasomal degradation and controls the activity of many signal transduction pathways. Ubiquitination occurs through the formation of an isopeptide bond between its C-terminus and a lysine residue in the target protein. This process can occur either as a monomer (monoubiquitin) or as a polymer (polyubiquitin chains), where the C-terminus of a chain extending ubiquitin becomes linked to the N-terminus (M1) or one of seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) within a substrate-bound ubiquitin molecule.Members of the ubiquitin-like protein family, such as small ubiquitin-related modifier 1, 2 and 3 (SUMO1, 2 and 3), have functions similar to ubiquitin, whereby they bind to target proteins as part of a post-translational modification system. SUMO can be covalently attached to proteins as a monomer or a lysine-linked polymer via an isopeptide bond. SUMO-1 regulates nuclear trafficking, formation of subnuclear structures, transcriptional activity, and protein stability by being conjugated to proteins such as RanGAP, PML, p53 and IκB-α. SUMO-2/-3 forms poly-(SUMO) chains and is conjugated to topoisomerase II and APP, regulating chromosomal segregation and cellular responses to environmental stress. Cellular location / Images Dot Blot Dot blot analysis of Anti-Diglycyl-Lysine Rabbit pAb (