anti-dicer mouse mab

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Recombinant Anti-Dicer Mouse mAb Clone Number: 13325 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: HERNA, KIAA0928 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a Conjugate Unconjugated Synonyms HERNA, KIAA0928 UniProt ID Q9UPY3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000 Human ICC/IF 1:50 – 1:250 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3′ overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: HepG2Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 220 kDaObserved band size: 250 kDa ICC/IF Cell line: HepG2Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:250Primary incubation condition: 4°C overnightNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Anti-Dicer Mouse mAb Clone Number: 13325 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: HERNA, KIAA0928 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a Conjugate Unconjugated Synonyms HERNA, KIAA0928 UniProt ID Q9UPY3 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:2000 Human ICC/IF 1:50 – 1:250 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3′ overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: 1: HepG2Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 220 kDaObserved band size: 250 kDa ICC/IF Cell line: HepG2Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:250Primary incubation condition: 4°C overnightNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with