anti-ddx3 rabbit mab

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Recombinant Rabbit Monoclonal Anti-DDX3 Rabbit mAb Clone Number: JMMR-3146 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: ATP-dependent RNA helicase DDX3X, CAP-Rf, DBX, DDX3X, HLP2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms ATP-dependent RNA helicase DDX3X, CAP-Rf, DBX, DDX3X, HLP2 UniProt ID O00571 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse ICC/IF 1:50 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DDX3, also named as DBX, DDX3X and HLP2, belongs to the DEAD box helicase family and DED1 subfamily. It is ATP-dependent RNA helicase. DDX3 acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 REV RNAs. It is also involved in HIV-1 replication. DDX3 interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. Cellular location Cell membrane, Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: NIH/3T3, 4: C6Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 73 kDaObserved band size: 73 kDa IHC-P Tissue: Human breast cancerSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-DDX3 Rabbit mAb Clone Number: JMMR-3146 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: ATP-dependent RNA helicase DDX3X, CAP-Rf, DBX, DDX3X, HLP2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms ATP-dependent RNA helicase DDX3X, CAP-Rf, DBX, DDX3X, HLP2 UniProt ID O00571 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:100 – 1:500 Human, Mouse ICC/IF 1:50 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DDX3, also named as DBX, DDX3X and HLP2, belongs to the DEAD box helicase family and DED1 subfamily. It is ATP-dependent RNA helicase. DDX3 acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 REV RNAs. It is also involved in HIV-1 replication. DDX3 interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. Cellular location Cell membrane, Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: NIH/3T3, 4: C6Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 73 kDaObserved band size: 73 kDa IHC-P Tissue: Human breast cancerSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with