anti-ddb1 mouse mab

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Recombinant Anti-DDB1 Mouse mAb Clone Number: 10828 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: XAP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms XAP1 UniProt ID Q16531 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DDB1 was initially implicated in the process of a specific type of DNA repair known as nucleotide excision repair. Since then, researchers have found that DDB1 primarily functions as a core component of the CUL4A- and CUL4B-based E3 ubiquitin ligase complexes. DDB1 serves as a bridge or adaptor protein which interacts with dozens of proteins known as DDB1 and CUL4-associated factors (DCAFs).[9] These DCAFs are often ubiquitin ligase substrates and regulate numerous essential processes in the cell including DNA repair (DDB2), DNA replication, chromatin remodeling (Cdt2) and more. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, 293, MCF-7, Raw264.7Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 127 kDaObserved band size: 127 kDa IHC-P Tissue: Human liver cancerSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-DDB1 Mouse mAb Clone Number: 10828 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: XAP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms XAP1 UniProt ID Q16531 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background DDB1 was initially implicated in the process of a specific type of DNA repair known as nucleotide excision repair. Since then, researchers have found that DDB1 primarily functions as a core component of the CUL4A- and CUL4B-based E3 ubiquitin ligase complexes. DDB1 serves as a bridge or adaptor protein which interacts with dozens of proteins known as DDB1 and CUL4-associated factors (DCAFs).[9] These DCAFs are often ubiquitin ligase substrates and regulate numerous essential processes in the cell including DNA repair (DDB2), DNA replication, chromatin remodeling (Cdt2) and more. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, 293, MCF-7, Raw264.7Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 127 kDaObserved band size: 127 kDa IHC-P Tissue: Human liver cancerSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and MousePolymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with