anti-cyr61 mouse mab

Vapor Pressure :
Recombinant Anti-CYR61 Mouse mAb Clone Number: JMMR-1936 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: IBP-10, GFBP-10, CCN1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms IBP-10, GFBP-10, CCN1 UniProt ID O00622 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CYR61(Cysteine-rich protein 61) is a member of CCN family, which is characterized primarily by its high cysteine content. CYR61 regulates diverse cellular events including cell proliferation, differentiation, angiogenesis, and extracellular matrix formation. CYR61 plays a role in the development or progression of various cancers, including breast, prostate, lung, and hepatocellular carcinoma. Notably, its role in promoting cancer progression appears to be context-dependent. For example, investigators have shown that overexpression of CYR61 was positively associated with invasiveness of breast cancer cell lines, whereas in primary prostate tumors, expression levels were inversely correlated with tumor aggressiveness. Cellular location Extracellular region or secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: MDA-MB-231 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 42 kDa Observed band size: 35 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: PC-3, 2: Saos-2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 42 kDa Observed band size: 35 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-CYR61 Mouse mAb Clone Number: JMMR-1936 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: IBP-10, GFBP-10, CCN1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms IBP-10, GFBP-10, CCN1 UniProt ID O00622 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CYR61(Cysteine-rich protein 61) is a member of CCN family, which is characterized primarily by its high cysteine content. CYR61 regulates diverse cellular events including cell proliferation, differentiation, angiogenesis, and extracellular matrix formation. CYR61 plays a role in the development or progression of various cancers, including breast, prostate, lung, and hepatocellular carcinoma. Notably, its role in promoting cancer progression appears to be context-dependent. For example, investigators have shown that overexpression of CYR61 was positively associated with invasiveness of breast cancer cell lines, whereas in primary prostate tumors, expression levels were inversely correlated with tumor aggressiveness. Cellular location Extracellular region or secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: MDA-MB-231 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 42 kDa Observed band size: 35 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: PC-3, 2: Saos-2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 42 kDa Observed band size: 35 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with