anti-cullin 1 mouse mab

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Recombinant Anti-Cullin 1 Mouse mAb Clone Number: JMMR-2681 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: CUL-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms CUL-1 UniProt ID Q13616 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cullin1 (CUL1) belongs to the cullin family proteins that are scaffold proteins for the Ring finger type E3 ligases. Human has seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Cullin1 is the mammalian homolog of Cdc53 from yeast. CUL1 has been shown to mediate ubiquitin dependent degradation of p21 Waf1/Cip1, cyclin D and IκBα. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. Cellular location Cytosol, nucleoplasm, plasma membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: U-87 MG, 2: IMR-32, 3: NIH/3T3, 4: Mouse brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 90 kDa Observed band size: 90 kDa :
Recombinant Anti-Cullin 1 Mouse mAb Clone Number: JMMR-2681 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: CUL-1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms CUL-1 UniProt ID Q13616 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cullin1 (CUL1) belongs to the cullin family proteins that are scaffold proteins for the Ring finger type E3 ligases. Human has seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Cullin1 is the mammalian homolog of Cdc53 from yeast. CUL1 has been shown to mediate ubiquitin dependent degradation of p21 Waf1/Cip1, cyclin D and IκBα. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. Cellular location Cytosol, nucleoplasm, plasma membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: U-87 MG, 2: IMR-32, 3: NIH/3T3, 4: Mouse brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 90 kDa Observed band size: 90 kDa