anti-ctcf mouse mab

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Recombinant Anti-CTCF Mouse mAb Clone Number: JMMR-3603 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor UniProt ID P49711 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CCCTC-binding factor (CTCF) and its paralog, the Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved transcription factors that regulate transcriptional activation and repression, insulator function, and imprinting control regions (ICRs). CTCF is ubiquitously expressed and contributes to transcriptional regulation of cell-growth regulated genes, including c-myc, p19/ARF, p16/INK4A, BRCA1, p53, p27, E2F1, and TERT. The various functions of CTCF are regulated by at least two different post-translational modifications. Poly (ADP-ribosyl) ation of CTCF is required for insulator function. Phosphorylation of Ser612 by protein kinase CK2 facilitates a switch of CTCF from a transcriptional repressor to an activator at the c-myc promoter. Moreover, CTCF mutations or deletions have been found in many breast, prostate, and Wilms tumors. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: HepG2, 3: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 140 kDa Observed band size: 140 kDa IHC-P Tissue: Human colon cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-CTCF Mouse mAb Clone Number: JMMR-3603 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor UniProt ID P49711 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background CCCTC-binding factor (CTCF) and its paralog, the Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved transcription factors that regulate transcriptional activation and repression, insulator function, and imprinting control regions (ICRs). CTCF is ubiquitously expressed and contributes to transcriptional regulation of cell-growth regulated genes, including c-myc, p19/ARF, p16/INK4A, BRCA1, p53, p27, E2F1, and TERT. The various functions of CTCF are regulated by at least two different post-translational modifications. Poly (ADP-ribosyl) ation of CTCF is required for insulator function. Phosphorylation of Ser612 by protein kinase CK2 facilitates a switch of CTCF from a transcriptional repressor to an activator at the c-myc promoter. Moreover, CTCF mutations or deletions have been found in many breast, prostate, and Wilms tumors. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: HepG2, 3: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 140 kDa Observed band size: 140 kDa IHC-P Tissue: Human colon cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with