anti-crotonyl-histone h4 (lys16) mouse mab

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Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H4 (Lys16) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IP IHC-P Reactivity: Human, Mouse, Rat, Africangreenmonkey Synonyms: H4K16cr Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K16cr UniProt ID P62805 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human，Mouse，Rat, African green monkey IP 1:25 Human IHC-P 1:200-1:1000 Human，Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H4 at Lys16 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: HeLa, C2C12, COS-7Protein loading quantity: 20 μgExposure time: 10 sPredicted MW: 11 kDaObserved MW: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: JK, N2a, BRL, H1299Protein loading quantity: 20 μgExposure time: 30 sPredicted MW: 11 kDaObserved MW: 11 kDa IP IP of HeLa+Crotonic acid (100ng/ml, 18hr) cells extractsIP ab incubation condition: :
Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H4 (Lys16) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IP IHC-P Reactivity: Human, Mouse, Rat, Africangreenmonkey Synonyms: H4K16cr Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K16cr UniProt ID P62805 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human，Mouse，Rat, African green monkey IP 1:25 Human IHC-P 1:200-1:1000 Human，Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H4 at Lys16 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: HeLa, C2C12, COS-7Protein loading quantity: 20 μgExposure time: 10 sPredicted MW: 11 kDaObserved MW: 11 kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Mouse IgG H&L (HRP)Lysate: JK, N2a, BRL, H1299Protein loading quantity: 20 μgExposure time: 30 sPredicted MW: 11 kDaObserved MW: 11 kDa IP IP of HeLa+Crotonic acid (100ng/ml, 18hr) cells extractsIP ab incubation condition: