anti-crotonyl-histone h3 (lys9) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Crotonyl-histone H3 (Lys9) Rabbit mAb Clone Number: 2H2L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K9cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K9cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys9) peptide MW (kDa) 15 Specificity Anti-Crotonyl-histone H3 (Lys9) Rabbit mAb detects histone H3 only when it is Crotonylated at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:50 – 1:100 Human ChIP 12 μg/5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys9 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1: 10000 Primary Ab incubation condition: 2 hours at room temperatureImmunogen peptide quantity: 4 ng, 16 ng, 64 ngExposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1: 2000 Primary Ab incubation condition: 2 hours at room temperature Lysate: (-) HeLa, (+)HeLa+crotonic acid (100 ng/ml, 18hr) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 17 kDa Observed band size: 17 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1: 2000 Primary Ab incubation condition: 2 hours at room temperature Lysate: N2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 17 kDa Observed band size: 17 kDa IHC-P Tissue: Human liver cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1: 1000 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Crotonyl-histone H3 (Lys9) Rabbit mAb Clone Number: 2H2L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K9cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K9cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys9) peptide MW (kDa) 15 Specificity Anti-Crotonyl-histone H3 (Lys9) Rabbit mAb detects histone H3 only when it is Crotonylated at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:50 – 1:100 Human ChIP 12 μg/5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys9 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1: 10000 Primary Ab incubation condition: 2 hours at room temperatureImmunogen peptide quantity: 4 ng, 16 ng, 64 ngExposure time: 60 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1: 2000 Primary Ab incubation condition: 2 hours at room temperature Lysate: (-) HeLa, (+)HeLa+crotonic acid (100 ng/ml, 18hr) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 17 kDa Observed band size: 17 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1: 2000 Primary Ab incubation condition: 2 hours at room temperature Lysate: N2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 17 kDa Observed band size: 17 kDa IHC-P Tissue: Human liver cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1: 1000 Primary Ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Description: The brown color represents the positive signal observed with