anti-crotonyl-histone h3 (lys4) mouse mab

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Anti-Crotonyl-Histone H3 (Lys4) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K4cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K4cr UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys4may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: (-) HeLa, (+) HeLa+Sodium crotonylate (30 mM, 4 hours) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 17 kDa Observed band size: 17 kDa ChIP Cell type: HeLa+Crotonic acid (10 mM, 15 h) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, RPL30 Exon 3, LDHA, FOXO3a-promoter, FOXO3a-downstream, RAB20, TUBBP10 and LDHA-promoter. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-Crotonyl-Histone H3 (Lys4) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K4cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K4cr UniProt ID P68431 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ChIP 6 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys4may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: (-) HeLa, (+) HeLa+Sodium crotonylate (30 mM, 4 hours) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 17 kDa Observed band size: 17 kDa ChIP Cell type: HeLa+Crotonic acid (10 mM, 15 h) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, RPL30 Exon 3, LDHA, FOXO3a-promoter, FOXO3a-downstream, RAB20, TUBBP10 and LDHA-promoter. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.