anti-crotonyl-histone h3 (lys36) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys36) Rabbit mAb Clone Number: PA-184-23 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: H3K36cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K36cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys36) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is crotonylated at Lys36, but not the acetylated, butyrylated, 2-hydroxyisobutyrylated and theunmodified H3 peptide at Lys36 Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human ICC/IF 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation,phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12,16 and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 60s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7, (+): MCF-7 + Sodium Butyrate (50mM, 24h) + Trichostatin A (500ng-ml, 4h)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa ICC/IF Cell line: (A) HeLa, (B) HeLa + Crotonic acid (10mM, 15hr)Fixation: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (red) Description: Color green is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys36) Rabbit mAb Clone Number: PA-184-23 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: H3K36cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K36cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys36) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is crotonylated at Lys36, but not the acetylated, butyrylated, 2-hydroxyisobutyrylated and theunmodified H3 peptide at Lys36 Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human ICC/IF 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation,phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12,16 and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 60s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7, (+): MCF-7 + Sodium Butyrate (50mM, 24h) + Trichostatin A (500ng-ml, 4h)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa ICC/IF Cell line: (A) HeLa, (B) HeLa + Crotonic acid (10mM, 15hr)Fixation: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (red) Description: Color green is the positive signal for