Product Name :
anti-crotonyl-histone h3 (lys27) rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H3K27cr
UniProt ID :
P68431
Immunogen:
Crotonylated human histone H3 (Lys27) peptide
MW (kDa) :
15
Specificity:
Anti-Crotonyl-Histone H3 (Lys27) Rabbit mAb detects histone H3 when it is crotonylated at Lys27.
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Lysine crotonylation is a newly-identified histone post-translational modification that has come to light through integrated proteomic approaches and elaborate biochemistry analyses. Lysine crotonylation has been found to be evolutionarily conserved across multiple species, spanning both prokaryotes and eukaryotes, and occurs in a wide range of proteins, incuding histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by exerting its effects on chromatin structure and epigenetic pathways. Cellular location Nucleus
Images :
Dot Blot Peptide amount: 1ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K27 crotonyl. Lane 2: H3K27 butyryl. Lane 3: H3K9 crotonyl.Dot 4: H3K27 acetyl. Dot 5: H3K27 2-hydroxyisobutyryl. Dot 6: H3K9 butyryl.Dot 7: H3K27 β-hydroxybutyryl. Dot 8: H3K9 acetyl. Dot 9: H3K9 crotonyl.Dot 10: H3K9 β-hydroxybutyryl. Dot 11: H3K9 2-hydroxyisobutyryl. Dot 12: H3K23 crotonyl. Dot 13: H3K27 unmodified. WB Lysates: (-): HeLa cells; (+): HeLa cells + crotonic acid (10 mM, 15 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds Predicted band size: 15 kDa Observed band size: 15 kDa Lysates: (-): 293T cells; (+): 293T cells + crotonic acid (10 mM, 15 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Exposure time: 30 secondsPredicted band size: 15 kDaObserved band size: 15 kDa ICC/IF Samples: (A) HeLa cells; (B) HeLa cells + crotonic acid (10 mM, 15 hours) Fixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Counter stain: Tubulin (red) Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys27) Rabbit mAb Clone Number: PF
Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys27) Rabbit mAb Clone Number: PF
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
TSG101 Rabbit mAb web
Oleclumab Autophagy
ATF4 Antibody (YA605): ATF4 Antibody (YA605) is a non-conjugated and Rabbit origined monoclonal antibody about 39 kDa, targeting to ATF4. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse, Rat.
