anti-crotonyl-histone h3 (lys18) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys18) Rabbit mAb Clone Number: JMMR-4101-VH Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: H3K18cr Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc.Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: HeLa + Crotonic acid (10mM, 15h) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa :
Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys18) Rabbit mAb Clone Number: JMMR-4101-VH Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: H3K18cr Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc.Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: HeLa + Crotonic acid (10mM, 15h) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa