anti-crotonyl-histone h3 (lys18) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys18) Rabbit mAb Clone Number: B1169/344-H1L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H3K18cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is crotonylated at Lys18, but not the H3 peptide crotonylatd at Lys14 and Lys23; the H3 peptide acetylated, butyrylated or propinylated at Lys14, Lys18 and Lys23 and the unmodified peptide at Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat ICC/IF 1:200-1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc.Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers.Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa + Crotonic acid (10mM, 15h)Protein loading amount: 20 μg Exposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Neuro-2a, (+): Neuro-2a + Crotonic acid (10mM, 15h)Protein loading amount: 20 μg Exposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): C6, (+): C6 + Crotonic acid (10mM, 15h)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa ICC/IF Cell line: (A) 293T, (B) 293T + Crotonic acid (10mM, 15hr)Fixation: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (red) Description: Color green is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Crotonyl-Histone H3 (Lys18) Rabbit mAb Clone Number: B1169/344-H1L1 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse, Rat Synonyms: H3K18cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is crotonylated at Lys18, but not the H3 peptide crotonylatd at Lys14 and Lys23; the H3 peptide acetylated, butyrylated or propinylated at Lys14, Lys18 and Lys23 and the unmodified peptide at Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:2000-1:10000 Human, Mouse, Rat ICC/IF 1:200-1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc.Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers.Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa + Crotonic acid (10mM, 15h)Protein loading amount: 20 μg Exposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Neuro-2a, (+): Neuro-2a + Crotonic acid (10mM, 15h)Protein loading amount: 20 μg Exposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): C6, (+): C6 + Crotonic acid (10mM, 15h)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa ICC/IF Cell line: (A) 293T, (B) 293T + Crotonic acid (10mM, 15hr)Fixation: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (blue)Counter stain: Tubulin (red) Description: Color green is the positive signal for