anti-crotonyl-histone h3 (lys18) mouse mab

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Anti-Crotonyl-Histone H3 (Lys18) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Anti-Crotonyl-Histone H3 (Lys18) Mouse mAb detects histone H3 only when it is crotonylated at Lys18. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:1000 – 1:5000 Human, Mouse, Rat ChIP 6 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K18cr, Lane 2: H3K9cr, Lane 3: H3K14me1, Lane 4: H3K18prLane 5: H3K18bu, Lane 6: H3K18bhb, Lane 7: H3K18me1, Lane 8: H3K23crLane 9: H3K27cr, Lane 10: H3K36cr, Lane 11: H3K56cr, Lane 12: H3K18un WB Lysates: (-): HeLa cells; (+): HeLa cells + crotonic acid (100 ng/ml, 18 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa Lysates: Jurkat, BRL cells Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human kidneySection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Anti-Crotonyl-Histone H3 (Lys18) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K18cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18cr UniProt ID P68431 Immunogen Crotonylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Anti-Crotonyl-Histone H3 (Lys18) Mouse mAb detects histone H3 only when it is crotonylated at Lys18. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:1000 – 1:5000 Human, Mouse, Rat ChIP 6 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided below.Lane 1: H3K18cr, Lane 2: H3K9cr, Lane 3: H3K14me1, Lane 4: H3K18prLane 5: H3K18bu, Lane 6: H3K18bhb, Lane 7: H3K18me1, Lane 8: H3K23crLane 9: H3K27cr, Lane 10: H3K36cr, Lane 11: H3K56cr, Lane 12: H3K18un WB Lysates: (-): HeLa cells; (+): HeLa cells + crotonic acid (100 ng/ml, 18 hours) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa Lysates: Jurkat, BRL cells Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa IHC-P Tissue: Human kidneySection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with