anti-crotonyl-histone h2b (lys20) mouse mab

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Anti-Crotonyl-Histone H2B (Lys20) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat, Monkey Synonyms: H2BK20cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK20cr UniProt ID P62807 Immunogen MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey ChIP 4 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H2B at Lys20 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, C2C12, COS-7, rH2BProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa ChIP Cell type: HeLa+Crotonic acid (10mM 15h)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 12 ugBeads type and amount per IP: 50 μl of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 1 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, RAB20, RPL30 Exon 3, and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-Crotonyl-Histone H2B (Lys20) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: Human, Mouse, Rat, Monkey Synonyms: H2BK20cr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK20cr UniProt ID P62807 Immunogen MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey ChIP 4 μg/5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H2B at Lys20 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, C2C12, COS-7, rH2BProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa ChIP Cell type: HeLa+Crotonic acid (10mM 15h)Cross-linking conditions: No cross-linkingAmount of chromatin per IP: 5×106 cellsAmount of Ab per IP: 12 ugBeads type and amount per IP: 50 μl of Protein A/G MagBeadsDescription: Chromatin immunoprecipitations were performed with 1 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS region, RAB20, RPL30 Exon 3, and FOXO3a-downstream regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.