Product Name :
anti-clathrin heavy chain mouse mab
Isotype :
IgG1/C-lambda
Conjugate :
Unconjugated
Synonyms:
CLTC, CLH17, CLTCL2
UniProt ID :
Q00610
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. Cellular location Cytoskeleton
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightLysate: 1: HeLa, 2: SH-SY5Y, 3: Jurkat, 4: NIH/3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 190 kDaObserved band size: 190 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Anti-Clathrin Heavy Chain Mouse mAb Clone Number: 13484 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: CLTC, CLH17, CLTCL2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/C-lambda Conjugate Unconjugated Synonyms CLTC, CLH17, CLTCL2 UniProt ID Q00610 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:200 – 1:1000 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. Cellular location Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightLysate: 1: HeLa, 2: SH-SY5Y, 3: Jurkat, 4: NIH/3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 190 kDaObserved band size: 190 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Recombinant Anti-Clathrin Heavy Chain Mouse mAb Clone Number: 13484 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: CLTC, CLH17, CLTCL2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/C-lambda Conjugate Unconjugated Synonyms CLTC, CLH17, CLTCL2 UniProt ID Q00610 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:200 – 1:1000 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. Cellular location Cytoskeleton Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightLysate: 1: HeLa, 2: SH-SY5Y, 3: Jurkat, 4: NIH/3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 190 kDaObserved band size: 190 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
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