anti-citrulline-histone h2a (arg3) rabbit pab

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Anti-Citrulline-Histone H2A (Arg3) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2AR3cit Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AR3cit UniProt ID P04908 Immunogen Citrullinated human histone H2A (Arg3) peptide MW (kDa) 14 Specificity Anti-Citrulline-Histone H2A (Arg3) Rabbit pAb detects histone H2A only when it is citrullinated at Arg3. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein citrullination is an emerging PTM that results from the conversion of peptidyl arginine to peptidyl citrulline. Due to the exchange of an imine for a carbonyl group, this reaction is referred to as deimination. This PTM is catalyzed by the calcium-regulated peptidylarginine deiminases (PAD). Dysregulated PAD expression and aberrant protein citrullination have been implicated in numerous human diseases such as osteosarcoma, colorectal cancer, esophagus cancer and breast cancer. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HL-60; (+): HL-60 + calcium ionophore A (4 μM, 15 minutes) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 14 kDa Observed band size: 14 kDa :
Anti-Citrulline-Histone H2A (Arg3) Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2AR3cit Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AR3cit UniProt ID P04908 Immunogen Citrullinated human histone H2A (Arg3) peptide MW (kDa) 14 Specificity Anti-Citrulline-Histone H2A (Arg3) Rabbit pAb detects histone H2A only when it is citrullinated at Arg3. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein citrullination is an emerging PTM that results from the conversion of peptidyl arginine to peptidyl citrulline. Due to the exchange of an imine for a carbonyl group, this reaction is referred to as deimination. This PTM is catalyzed by the calcium-regulated peptidylarginine deiminases (PAD). Dysregulated PAD expression and aberrant protein citrullination have been implicated in numerous human diseases such as osteosarcoma, colorectal cancer, esophagus cancer and breast cancer. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HL-60; (+): HL-60 + calcium ionophore A (4 μM, 15 minutes) Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 14 kDa Observed band size: 14 kDa