anti-citrate synthase mouse mab

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Recombinant Anti-Citrate Synthase Mouse mAb Clone Number: JMMR-3604 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: CS Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms CS UniProt ID O75390 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Citrate synthase (CS) is a mitochondrial enzyme that catalyzes the first and rate-limiting reaction of the citric acid cycle. The enzyme is responsible for the conversion of oxaloacetate and acetyl-CoA to citrate and CoA. Research studies show that CS knockdown HeLa cells are more malignant than vector-transfected cells from an in vivo xenograft model, which suggests a direct link between the Warburg Effect and tumor growth. Intervertebral disc degeneration, a growing health problem, has been linked to changes in citrate synthase activity. Cellular location Mitochondrion matrix Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: Caco-2, 3: NIH/3T3, 4: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 52 kDa Observed band size: 45 kDa IHC-P Tissue: Human thyroid carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-Citrate Synthase Mouse mAb Clone Number: JMMR-3604 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: CS Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms CS UniProt ID O75390 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 Human, Mouse ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Citrate synthase (CS) is a mitochondrial enzyme that catalyzes the first and rate-limiting reaction of the citric acid cycle. The enzyme is responsible for the conversion of oxaloacetate and acetyl-CoA to citrate and CoA. Research studies show that CS knockdown HeLa cells are more malignant than vector-transfected cells from an in vivo xenograft model, which suggests a direct link between the Warburg Effect and tumor growth. Intervertebral disc degeneration, a growing health problem, has been linked to changes in citrate synthase activity. Cellular location Mitochondrion matrix Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: Caco-2, 3: NIH/3T3, 4: PC-12 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 52 kDa Observed band size: 45 kDa IHC-P Tissue: Human thyroid carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with