anti-chd4 rabbit pab

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Anti-CHD4 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human Synonyms: ATP-dependent helicase CHD4, Mi2-beta Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms ATP-dependent helicase CHD4, Mi2-beta UniProt ID Q14839 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:3000 Human IHC-P 1:400-1:2000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity. Cellular location Nucleus, Centrosome Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:3000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: HeLa, 3: HEK-293Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 218 kDaObserved band size: 260 kDa IHC-P Tissue: Human breastSection type: Formalin-fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-CHD4 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human Synonyms: ATP-dependent helicase CHD4, Mi2-beta Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms ATP-dependent helicase CHD4, Mi2-beta UniProt ID Q14839 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:3000 Human IHC-P 1:400-1:2000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity. Cellular location Nucleus, Centrosome Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:3000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF-7, 2: HeLa, 3: HEK-293Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 218 kDaObserved band size: 260 kDa IHC-P Tissue: Human breastSection type: Formalin-fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:2000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with