anti-chd3 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-CHD3 Rabbit mAb Clone Number: 13397 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Rat Synonyms: Mi2-alpha, hZFH Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Mi2-alpha, hZFH UniProt ID Q12873 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Chromodomain-helicase-DNA-binding protein 3(CHD3) is an enzyme that is encoded by the CHD3 gene in human. This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD3 is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Mutations in CHD3 gene cause a neurodevelopmental syndrome with macrocephaly and impaired speech and language. Cellular location Nucleus, Centrosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: C6, 2: HeLa Protein loading quantity: 20 μg Exposure time: 1: 60 s, 2: 30 secondsPredicted band size: 227 kDa Observed band size: 260 kDa IHC-P Tissue: Human breast Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-CHD3 Rabbit mAb Clone Number: 13397 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Rat Synonyms: Mi2-alpha, hZFH Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Mi2-alpha, hZFH UniProt ID Q12873 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Chromodomain-helicase-DNA-binding protein 3(CHD3) is an enzyme that is encoded by the CHD3 gene in human. This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD3 is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Mutations in CHD3 gene cause a neurodevelopmental syndrome with macrocephaly and impaired speech and language. Cellular location Nucleus, Centrosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: C6, 2: HeLa Protein loading quantity: 20 μg Exposure time: 1: 60 s, 2: 30 secondsPredicted band size: 227 kDa Observed band size: 260 kDa IHC-P Tissue: Human breast Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with