anti-acetyl-histone h2b (lys20) mouse mab

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Anti-Acetyl-Histone H2B (Lys20) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H2BK20ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK20ac UniProt ID P62807 Immunogen Acetylated human histone H2B (Lys20) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2B (Lys20) Mouse mAb detects histone H2B only when it is Acetylated at Lys20. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 – 1:150 Human ChIP 4 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15 and 36; H2B is primarily acetylated at Lys5, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in cancers and other diseases. Therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) Hela + sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa IHC-P Tissue: Human testisSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-Acetyl-Histone H2B (Lys20) Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H2BK20ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2BK20ac UniProt ID P62807 Immunogen Acetylated human histone H2B (Lys20) peptide MW (kDa) 14 Specificity Anti-Acetyl-Histone H2B (Lys20) Mouse mAb detects histone H2B only when it is Acetylated at Lys20. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 – 1:150 Human ChIP 4 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15 and 36; H2B is primarily acetylated at Lys5, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in cancers and other diseases. Therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1 ng, 4 ng, 16 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: (-) HeLa, (+) Hela + sodium butyrate (30mM, 4 hours)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14 kDa IHC-P Tissue: Human testisSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with