Product Name :
anti-cdc25c rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
M-phase inducer phosphatase 3
UniProt ID :
P30307
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Cdc25C is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis. Cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint. When phosphorylated at Ser216, Cdc25C binds to members of the 14-3-3 family of proteins, sequestering Cdc25C in the cytoplasm and thereby preventing premature mitosis. The checkpoint kinases Chk1 and Chk2 phosphorylate Cdc25C at Ser216 in response to DNA damage. Cellular location Nucleus
Images :
WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hoursat room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa; 2: K562; 3: HEK293; 4: NIH/3T3; 5: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 53 kDa Observed band size: 53 kDa
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Cdc25C Rabbit mAb Clone Number: JRMR-11033-7 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: M-phase inducer phosphatase 3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms M-phase inducer phosphatase 3 UniProt ID P30307 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cdc25C is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis. Cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint. When phosphorylated at Ser216, Cdc25C binds to members of the 14-3-3 family of proteins, sequestering Cdc25C in the cytoplasm and thereby preventing premature mitosis. The checkpoint kinases Chk1 and Chk2 phosphorylate Cdc25C at Ser216 in response to DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hoursat room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa; 2: K562; 3: HEK293; 4: NIH/3T3; 5: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 53 kDa Observed band size: 53 kDa
Recombinant Rabbit Monoclonal Anti-Cdc25C Rabbit mAb Clone Number: JRMR-11033-7 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: M-phase inducer phosphatase 3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms M-phase inducer phosphatase 3 UniProt ID P30307 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cdc25C is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis. Cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint. When phosphorylated at Ser216, Cdc25C binds to members of the 14-3-3 family of proteins, sequestering Cdc25C in the cytoplasm and thereby preventing premature mitosis. The checkpoint kinases Chk1 and Chk2 phosphorylate Cdc25C at Ser216 in response to DNA damage. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hoursat room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa; 2: K562; 3: HEK293; 4: NIH/3T3; 5: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 53 kDa Observed band size: 53 kDa
Related websites: https://www.medchemexpress.com/antibodies.html
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