anti-acetyl-histone h2a (lys9) mouse mab

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Anti-Acetyl-Histone H2A (Lys9) Mouse mAb Clone Number: 33-2-2 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2AK9ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms H2AK9ac UniProt ID P04908 Immunogen Acetylated human histone H2A (Lys9) peptide MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases(HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2-5: 64 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB locking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): HeLa, (+): HeLa+ sodium butyrate (30mM, 4 hours) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 14 KDa Observed band size: 14 KDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Lysate: 1: Neuro-2a, 2: BRL, 3: Mouse brain, 4: Rat brain Protein loading quantity: 20 ug Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa :
Anti-Acetyl-Histone H2A (Lys9) Mouse mAb Clone Number: 33-2-2 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: H2AK9ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1 Conjugate Unconjugated Synonyms H2AK9ac UniProt ID P04908 Immunogen Acetylated human histone H2A (Lys9) peptide MW (kDa) 14 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases(HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 1: 1ng, 4 ng, 16 ng, 64 ng, 2-5: 64 ng Exposure time: 60 secondsThe list of peptides is included in the table below. WB locking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): HeLa, (+): HeLa+ sodium butyrate (30mM, 4 hours) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 14 KDa Observed band size: 14 KDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Lysate: 1: Neuro-2a, 2: BRL, 3: Mouse brain, 4: Rat brain Protein loading quantity: 20 ug Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa