anti-caspase-7 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Caspase-7 Rabbit mAb Clone Number: JRMR-10984-20 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Mouse, Rat Synonyms: CASP-7, Apoptotic protease Mch-3, Cysteine protease LICE2, Lice2, Mch3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CASP-7, Apoptotic protease Mch-3, Cysteine protease LICE2, Lice2, Mch3 UniProt ID P97864 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse, Rat IHC-P 1:200 – 1:1000 Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Caspase-7, also named as CMH-1, Mch3 and ICE-LAP3, has been identified as a major contributor to the execution of apoptosis. Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as ADP-ribose polymerase and PARP. During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits. Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: mouse spleen; 2: rat spleen Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 34 kDa Observed band size: 34 kDa IHC-P Tissue: Mouse colon Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Caspase-7 Rabbit mAb Clone Number: JRMR-10984-20 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Mouse, Rat Synonyms: CASP-7, Apoptotic protease Mch-3, Cysteine protease LICE2, Lice2, Mch3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CASP-7, Apoptotic protease Mch-3, Cysteine protease LICE2, Lice2, Mch3 UniProt ID P97864 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse, Rat IHC-P 1:200 – 1:1000 Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Caspase-7, also named as CMH-1, Mch3 and ICE-LAP3, has been identified as a major contributor to the execution of apoptosis. Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as ADP-ribose polymerase and PARP. During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits. Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: mouse spleen; 2: rat spleen Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 34 kDa Observed band size: 34 kDa IHC-P Tissue: Mouse colon Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with